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eclipse te2000-e inverted fluorescence microscope  (Nikon)

 
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    Nikon eclipse te2000-e inverted fluorescence microscope
    Eclipse Te2000 E Inverted Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eclipse te2000-e inverted fluorescence microscope/product/Nikon
    Average 90 stars, based on 1 article reviews
    eclipse te2000-e inverted fluorescence microscope - by Bioz Stars, 2026-02
    90/100 stars

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    Streptococcus mutans biofilm pH displays two fluorescent peaks over time. ( A ) S. mutans biofilm pH analyzed with pHrodo red exhibits continuously increasing fluorescence with two fluorescent peaks over 30 hours. ( B ) S. mutans GFP cell fluorescence exhibits a sharp preliminary peak intensity before waning followed by a slow recovery. Time-lapse data were collected using a Zeiss Cell Discoverer7 (CD7) microscope with 50× PlanApp Water immersion lens. Mean data were collected for 41 z-stacks taken per hour per image site over three independent experiments performed in duplicate with three to four image sites per well. Source of S. mutans G09 and G18 is Child 5 (C-232).

    Journal: mSphere

    Article Title: Intraspecies interactions of Streptococcus mutans impact biofilm architecture and virulence determinants in childhood dental caries

    doi: 10.1128/msphere.00778-23

    Figure Lengend Snippet: Streptococcus mutans biofilm pH displays two fluorescent peaks over time. ( A ) S. mutans biofilm pH analyzed with pHrodo red exhibits continuously increasing fluorescence with two fluorescent peaks over 30 hours. ( B ) S. mutans GFP cell fluorescence exhibits a sharp preliminary peak intensity before waning followed by a slow recovery. Time-lapse data were collected using a Zeiss Cell Discoverer7 (CD7) microscope with 50× PlanApp Water immersion lens. Mean data were collected for 41 z-stacks taken per hour per image site over three independent experiments performed in duplicate with three to four image sites per well. Source of S. mutans G09 and G18 is Child 5 (C-232).

    Article Snippet: To determine biofilm pH, wells were manually surveyed and 2D representative images collected at 10× using a fluorescent microscope (Nikon TE2000-E inverted scope).

    Techniques: Fluorescence, Microscopy